19 7월 QTLs controlling SW, SD, SL, and you will SS have been assessed playing with phenotypic rating and SSR marker studies regarding the BC
1P1 generationsposite interval mapping was used for QTL detection. The window size was set at 5.0 cM and the walk speed at 1.0 cM. Genome-wide significance thresholds were estimated by 1000 permutations to declare significant QTLs with a type-I error rate of 0.05 (Churchill and Doerge, 1994). Finally, QTLs were as presented by WinQTLCart dos.5 (Wang et al., 2012).
Genetic analysis off trend seed and you may strict-placenta fruit traits.
Fifteen F1 individuals, generated by crossing MR-1 with ‘Queen', showed glossy seeds and untight-placenta fruits. In the BC1P1 population, 63 of 134 individuals showed glossy seeds and 71 showed the wave seeds. Sixty-nine of 134 individuals showed untight-placenta fruits and 65 showed the tight-placenta fruits. Segregation ratios were confirmed to be 1:1 by ? 2 test. Wave seeds and tight-placenta plants were never observed in BC1P2 population.