28 6월 Regarding ease of polymorphism knowledge, the fresh new interspecific strategy brings an obvious advantage over the new RI approach
First, how many examples in several of the well-characterized interspecific boards ranges off more 2 hundred to just like the high as a whole thousand; a thousand examples brings the typical chart solution out-of 0
As the discussed in past times, it has been hard to discover RFLPs within progenitors off RI stresses. Additionally, this new identity from good RFLP between that set of RI progenitors is commonly perhaps not utilized for the analysis away from almost every other RI set. Ergo, whether or not RFLPs was basically uncovered, the full quantity of RI strains that is certainly reviewed is often quite minimal; it can be less than twenty-six and is rarely more 80. In contrast, the convenience off RFLP character involving the progenitors of your own interspecific get across is actually area of the impetus with the first the means to access so it mapping means. Also, you to definitely you desire simply pick one particular polymorphism to type the whole interspecific committee.
To your brand new PCR-based answers to polymorphism identification talked about into the point 8.step three, it is currently more straightforward to select differences between RI progenitor stresses. However, with these exact same ways, polymorphism personality between the interspecific progenitors is even smoother however.
That have a total for each-attempt sized you to definitely milligram away from DNA, one can possibly develop two hundred Southern area blots that will for each end up being probed multiple times
In terms of the resolution of genetic map that's acquired, the new interspecific means have plenty of gurus along side RI strategy.